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Coding
CapH

Part:BBa_K314015:Design

Designed by: Chris Eiben, Tomas Huber, David Zong   Group: iGEM10_Washington   (2010-10-08)


CapD_CP-F24H


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1489
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Point mutation at residue 24 on Foldit design from Phenylalanine to Histidine, Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel


Source

In-House Mutant

References